One-path NAD+ protocol, with exercise — experimental

 

 

Fission/mitophagy (morning on empty stomach)

Nicotinamide (NAD+) — 2g

Ribose (NAD+) — 2g

AMPK activators (Life Extension) — 1g

Fisetin (Sirt1 activator) — 100mg

 

1.5-2 hours later — gym

 

Repeat. 2-3 days total

 

Fusion/Biogenesis (morning, first day of fusion)

Stearic acid (fusion) — 5g

Leucine (biogenesis) — 5g

PQQ (biogenesis) — 20mg

Hydroxytyrosol (biogenesis & antioxidant) — 25mg

Vitamin B complex (commercial mix)

 

Fusion/Biogenesis (morning, second day of fusion)

Broccoli sprout extract with sulforaphane (fusion) — .5-1g

Leucine (biogenesis) — 5g

PQQ (biogenesis) — 20mg

Hydroxytyrosol (biogenesis & antioxidant) — 25mg

Vitamin B complex (commercial mix)

 

 

Notes:

 

1. AMPK activators — See Ampk phosphorylation of Ulk1 is required for targeting of mitochondria to lysosomes in exercise-induced mitophagy. In addition, it appears that the advantages of spermidine derive at least in part from its activation of AMPK —Spermidine coupled with exercise rescues skeletal muscle atrophy from D-gal-induced aging rats through enhanced autophagy and reduced apoptosis via AMPK-FOXO3a signal pathway. Spermidine is difficult to find so I haven’t included it in the protocol. 

2. Sirt1 activator — See Nicotinamide-induced mitophagy: event mediated by high NAD+/NADH ratio and SIRT1 protein activation. 

3. Nicotinamide is the same as niacinamide, while nicotinic acid is the same as niacin. 

4. Stearic has a long biological half life of 17 hours so I use it only on the first day of fusion. Sulforanphane has a much shorter half life.